Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: Relationships between lncRNA AC010761.9 expression (ΔCt value) and patient clinical pathologic factors and serum tumor markers

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA tissues by lncRNA expression chip assay. T cancer tissues, N matched non-cancer tissues. Cluster analyses from the six GA and their paired non-GA tissues lncRNA chip results showed that LncRNA AC010761.9 was over-expressed in GA tissues compared with that in the paired non-GA tissues (mean increased fold = 2.01 times, p < 0.05)

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA tissues by quantified RT-PCR measurement. T cancer tissues, N matched non-cancer tissues. T versus N, p < 0.01. The data were from 145 cases of GA. The higher the ΔCt values, the lower the lncRNA AC010761.9 expression. Data were obtained from three independent tests

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: LncRNA AC010761.9 was over-expressed in GA cell lines. The data were from three GA cell lines (MGC-803, BGC-823, and SGC-7901) and control cells (normal gastric cell line [GES-1]). GA cells versus control cells (all p < 0.05). The higher the ΔCt values, the lower the lncRNA AC010761.9 expression. Data were obtained from three independent tests

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Control, Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: Relationships between lncRNA AC010761.9 expression (ΔCt value) and patient clinical pathologic factors analyzed by univariate and multivariate

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Evaluation of the expression and clinical value of lncRNA AC010761.9 in human gastric adenocarcinoma

doi: 10.1186/s12957-017-1289-y

Figure Lengend Snippet: The expression of lncRNA AC010761.9 showed a positive correlation with the expression of TRAF4 mRNA. r = 0.385 and p < 0.01 were obtained by Pearson correlation analysis

Article Snippet: The results from the Human LncRNA Expression Profile Microarray V3.0 (Arraystar, Rockville, MD, USA) were obtained from the previous work and completed by Kang Chen Bio-tech (Shanghai, China) based on the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Schematic depicting the composition of circRNA_101237 (encoded by CDK8). circRNA, circular RNA; CDK8, cyclin-dependent kinase 8; chr, chromosome.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: CircRNA_101237 expression in HCC tissues. (A) Heatmap for differentially expressed circRNAs in HCC tumor tissues and the matched adjacent tissues (n=3). (B) RT-qPCR was performed to determine the expression of circRNA_101237 in tumor tissues (n=100) and adjacent tissues (n=100). (C) RT-qPCR was performed to assess the expression of circRNA_101237 in serum samples from patients with HCC (n=234) and healthy controls (n=120). (D) Kaplan-Meier survival curves were drawn to compare the survival of patients with high circRNA_101237 expression (n=148) and those with low circRNA_101237 expression (n=86). RT-qPCR, reverse transcription-quantitative PCR; HCC, hepatocellular carcinoma; circRNA_101237, circular RNA 101237; hsa, Homo sapiens.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Clinical association between serum circRNA_101237 levels and clinicopathological characteristics of patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Univariate analysis of prognostic factors for patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Multivariate analysis of independent prognostic factors for patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Expression of circRNA_101237 is associated with cisplatin resistance. (A) RT-qPCR was performed to assess the expression of circRNA_101237 in serum samples of patients with cisplatin-sensitive HCC (n=50) and those with cisplatin-resistant HCC (n=35). (B) RT-qPCR was performed to determine the expression of circRNA_101237 in liver cancer cells. * P<0.05 vs. HCCLM3 cells; #P<0.05, Huh7/DDP vs. Huh7. Huh/DDP, cisplatin-resistant Huh7 cells; RT-qPCR, reverse transcription-quantitative PCR; HCC, hepatocellular carcinoma; circRNA_101237, circular RNA 101237.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Changes in circRNA_101237 expression in hepatocellular carcinoma cell lines in response to cisplatin treatment. (A) Cells were treated for 48 h with cisplatin at 0, 0.5, 1 and 2 µg/ml. (B) Cells were treated with cisplatin at 1 µg/ml for 0, 12, 24 or 48 h. Reverse transcription-quantitative PCR was performed to assess the expression of circRNA_101237. *P<0.05 vs. controls. circRNA_101237, circular RNA 101237.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Communications Biology

Article Title: tRF16 affects NFKBIA stability and promotes osteoarthritis progression by regulating ALKBH5 expression in m6A-dependent manner

doi: 10.1038/s42003-025-08299-y

Figure Lengend Snippet: A , B Volcano and heat maps of smallRNAs differentially expressed in cartilage tissues of three normal subjects and three OA patients analyzed using Arraystar Human SmallRNA Expression Microarray; C flow diagram of a rat OA model established by meniscectomy. The illustration is created by Biorender.com; D microCT confirms arthritic symptoms in rats at 16 weeks postoperatively; E the Tb.N, Tb.sp and BV/TV value according to the micro-CT; F – H , HE, PAS, and SAFG staining to detect pathological structural alterations in the cartilage tissues of rat knee joints; I RT-qPCR to detect tRF16 expression in the cartilage tissues of rats at 0, 4, 8, 12, and 16 weeks postoperatively; J RT-qPCR to detect the mRNA levels of Aggrecan, COL2A1, MMP1, and MMP13 in the cartilage tissues of rats; K the correlation between tRF16 expression in the knee joints of OA rats at week 16 and the Mankin score. Each group contained 6-8 rats; the data were presented as dot and whsikers and statistically analyzed using Student t -test or 2-way ANOVA, followed by Tukey’s multiple comparison test for post hoc tests, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In the present study, we used Arraystar Human SmallRNA Expression Microarray to analyze the differentially expressed small RNA in cartilage tissue and observed significantly overexpressed tRF16 in OA patients.

Techniques: Expressing, Microarray, Micro-CT, Staining, Quantitative RT-PCR, Comparison

Journal: Non-coding RNA Research

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention

doi: 10.1016/j.ncrna.2023.11.012

Figure Lengend Snippet: Schematic illustration of the effect of circular RNAs (circRNAs) on intracranial aneurysm (IA) rupture. Current evidence strongly suggests a central role for endothelial dysfunction in the initiation and progression of IA. Post-subarachnoid hemorrhage (SAH), several early pathophysiological events can be commonly observed in blood-brain barrier (BBB) components, such as the endothelium (endothelial dysfunction). In results, post- SAH injuries can disrupt the integrity and function of the BBB . Both negative (red cross) and positive (green cross) regulation of circRNAs have been observed in this pathological cascade. The role of circRNAs is based on components: 1) strong role in endothelial cells (ECs) homeostasis; 2) regulation of barrier function and vascular tone; 3) associated with SAH and its complications; 4) correlates with clinical outcomes (Glasgow Coma Scale, the volume of SAH, modified Fisher scale, Hunt-Hess levels, and surgical type; 5) regulators of transcription/translation, sequesters of microRNA (miRNA)/RNA-binding proteins (RBPs), and biomarkers of IA.

Article Snippet: Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed.

Techniques: Modification, RNA Binding Assay

Journal: Non-coding RNA Research

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention

doi: 10.1016/j.ncrna.2023.11.012

Figure Lengend Snippet: Schematic illustration of circular RNAs (circRNAs) regulation mechanisms underlying vascular smooth muscle cells (VSMCs) phenotypic modulation, oxidative stress, and cell death in intracranial aneurysms (IAs). As can be seen from the figure, circRNAs play a role both in the development and progression of IA and in the inhibition of IA through the control of VSMC. However, some of them exhibit a double effect as circ_FOXO3 and circ_0020397.

Article Snippet: Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed.

Techniques: Inhibition

Journal: Non-coding RNA Research

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention

doi: 10.1016/j.ncrna.2023.11.012

Figure Lengend Snippet: Benefits of using cell free circular RNAs (circRNAs) as biomarkers.

Article Snippet: Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed.

Techniques:

Journal: Non-coding RNA Research

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention

doi: 10.1016/j.ncrna.2023.11.012

Figure Lengend Snippet: The studied cell free circular RNAs (circRNAs) are presented as non-invasive biomarkers in intracranial aneurysms (IAs).

Article Snippet: Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed.

Techniques:

Journal: Non-coding RNA Research

Article Title: Circular RNAs in intracranial aneurysms: Emerging roles in pathogenesis, diagnosis and therapeutic intervention

doi: 10.1016/j.ncrna.2023.11.012

Figure Lengend Snippet: Summary information on the role of circular RNAs (circRNAs) in the formation and development of intracranial aneurysms (IAs).

Article Snippet: Leveraging the power of the Arraystar human circRNAs microarray, differentially expressed circRNAs between patients with UIAs and RIAs were meticulously analyzed.

Techniques: Migration, Transformation Assay

Journal: Bioengineered

Article Title: Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis

doi: 10.1080/21655979.2022.2070975

Figure Lengend Snippet: Curcumol upregulates miR-7 expression in GC cells. a, miRNAs with differential expression in cells after curcumol treatment identified by microarray analysis; b, miR-7 expression in cells after 80 μM curcumol treatment examined by RT-qPCR (* p < 0.05; two-way ANOVA); c, miR-7 expression in tumor and the paired normal tissues evaluated by RT-qPCR (n = 33; * p < 0.05; the unpaired t test); d, correlation between miR-7 expression and the patient’s survival (* p < 0.05; Kaplan-Meier analysis); e, miR-7 expression in MKN45 and HGC27 cells after different doses of curcumol treatment examined by RT-qPCR (* p < 0.05, ** p < 0.01; two-way ANOVA).

Article Snippet: This reaction was performed at 37°C for 30 min. After that, the labeled RNA was hybridized with Human miRNA Expression Microarray V4.0 (Arraystar, Rockville, MD, USA) for 24 h. The gene expression data were obtained using a GeneChip TM Scanner 3000 7 G system (#00-0210, 2008, Thermo Fisher Scientific) and analyzed by the R Language Program (Version 3.6.3, R).

Techniques: Expressing, Quantitative Proteomics, Microarray, Quantitative RT-PCR